CS Antikörper

Produktnummer ABIN2856769

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CS in WB, IP, ICC, IF, IHC (p) und IHC (wm). Er zeigt eine Reaktivität gegenüber Human und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für CS Antikörper (ABIN2856769)

Target: CS (Citrate Synthase (CS))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Güteklasse: KO Validated

Produktdetails anti-CS Antikörper

Kreuzreaktivität: Huhn, Frosch, Human, Affe, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to Citrate synthetase (citrate synthase)
Citrate synthetase antibody [N2C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: mouse brain , rat brain , Rat muscle , 293T

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.65 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-CS Antikörper (ABIN2856769)

Ding, Rose, Sun, Wu, Chen, Lin, Yang, Chen, Lee, Xu, Tian, Akinwuntan, Zhao, Guan, Zhou, Chi: "MESH1 is a cytosolic NADPH phosphatase that regulates ferroptosis." in: Nature metabolism, Vol. 2, Issue 3, pp. 270-277, (2020) (PubMed).

Leandro, Violante, Argmann, Hagen, Dodatko, Bender, Zhang, Williams, Bachmann, Auwerx, Yu, Houten: "Mild inborn errors of metabolism in commonly used inbred mouse strains." in: Molecular genetics and metabolism, Vol. 126, Issue 4, pp. 388-396, (2019) (PubMed).

Kim, Men, Liang, Receno, Brutsaert, Korol, Heffernan, DeRuisseau: "Effects of long-term exposures to low iron and branched-chain amino acid containing diets on aging skeletal muscle of Fisher 344 × Brown Norway rats." in: Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme, Vol. 43, Issue 2, pp. 165-173, (2018) (PubMed).

Nam, Akie, Sanosaka, Craige, Kant, Keaney, Cooper: "Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression." in: Scientific reports, Vol. 7, Issue 1, pp. 2013, (2018) (PubMed).

Chang, Ulrich, Suliman, Piantadosi: "Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis." in: Free radical biology & medicine, Vol. 78, pp. 179-89, (2015) (PubMed).

Details zu CS

Target: CS (Citrate Synthase (CS))

Andere Bezeichnung: citrate synthase

Hintergrund: The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

Cellular Localization: Mitochondrion matrix

Molekulargewicht: 52 kDa

Gen-ID: 1431

UniProt: O75390