FH Antikörper

Produktnummer ABIN2856710

Der Kaninchen Polyklonal Anti-FH-Antikörper wurde für WB, IF, IHC (p) und ICC validiert. Er ist geeignet, FH in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für FH Antikörper (ABIN2856710)

Target: FH (Fumarate Hydratase (FH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Produktdetails anti-FH Antikörper

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Fumarate hydratase (fumarate hydratase)
Fumarate hydratase antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: mouse liver , rat liver , 293T

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.49 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-FH Antikörper (ABIN2856710)

Miettinen, Felisiak-Golabek, Wasag, Chmara, Wang, Butzow, Lasota: "Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases." in: The American journal of surgical pathology, Vol. 40, Issue 12, pp. 1661-1669, (2017) (PubMed).

Details zu FH

Target: FH (Fumarate Hydratase (FH))

Andere Bezeichnung: fumarate hydratase

Hintergrund: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Cellular Localization: Mitochondrion , Cytoplasm

Molekulargewicht: 55 kDa

Gen-ID: 2271

UniProt: P07954