RAG2 Antikörper (Center)

Produktnummer ABIN2856698

Der Kaninchen Polyklonal Anti-RAG2-Antikörper wurde für WB, IF, IHC (p) und ICC validiert. Er ist geeignet, RAG2 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für RAG2 Antikörper (Center) (ABIN2856698)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAG2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-RAG2 Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Maus, Schwein, Kaninchen, Ratte (Rattus), Rind (Kuh)

Kreuzreaktivität (Details): Mouse (85 %), Pig (90 %), Rabbit (91 %), Rat (86 %), Bovine (91 %)

Produktmerkmale: Rabbit Polyclonal antibody to RAG2 (recombination activating gene 2)
RAG2 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: Molt-4 , rat thymus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.37 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Andere Bezeichnung: RAG2

Hintergrund: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Cellular Localization: Nucleus

Molekulargewicht: 59 kDa

Gen-ID: 5897

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response