POLRMT Antikörper (Center)

Produktnummer ABIN2856044

Der Kaninchen Polyklonal Anti-POLRMT-Antikörper wurde für WB, IF, ICC, IHC (p) und CUT&RUN validiert. Er ist geeignet, POLRMT in Proben von Human und Maus zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für POLRMT Antikörper (Center) (ABIN2856044)

Target: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLRMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Produktdetails anti-POLRMT Antikörper

Bindungsspezifität: Center

Produktmerkmale: Rabbit Polyclonal antibody to mtRNA polymerase (polymerase (RNA) mitochondrial (DNA directed))
mtRNA polymerase antibody [N2N3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.31 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-POLRMT Antikörper (ABIN2856044)

Kasinathan, Henikoff: "Non-B-Form DNA Is Enriched at Centromeres." in: Molecular biology and evolution, Vol. 35, Issue 4, pp. 949-962, (2019) (PubMed).

Thakur, Henikoff: "Unexpected conformational variations of the human centromeric chromatin complex." in: Genes & development, Vol. 32, Issue 1, pp. 20-25, (2018) (PubMed).

Sobek, Dalla Rosa, Pommier, Bornholz, Kalfalah, Zhang, Wiesner, von Kleist-Retzow, Hillebrand, Schaal, Mielke, Christensen, Boege: "Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I." in: Nucleic acids research, Vol. 41, Issue 21, pp. 9848-57, (2014) (PubMed).

Details zu POLRMT

Target: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Andere Bezeichnung: MtRNA Polymerase

Hintergrund: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

Cellular Localization: Mitochondrion

Molekulargewicht: 139 kDa

Gen-ID: 5442