Proteasome 20S alpha 4 (Center) Antikörper

Produktnummer ABIN2855988

Produktdetails

Target: Proteasome 20S alpha 4

Bindungsspezifität: Center

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Kreuzreaktivität: Schwein, Rhesusaffen, Rind (Kuh)

Kreuzreaktivität (Details): Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to Proteasome 20S alpha 4 (proteasome (prosome, macropain) subunit, alpha type, 4)
Proteasome 20S alpha 4 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: HeLa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: Proteasome 20S alpha 4

Hintergrund: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Nucleus

Molekulargewicht: 29 kDa

Gen-ID: 5685