CKMT1B Antikörper

Produktnummer ABIN2855669

Der Kaninchen Polyklonal Anti-CKMT1B-Antikörper wurde für WB, IHC (p) und IHC (wm) validiert. Er ist geeignet, CKMT1B in Proben von Human zu detektieren.

Kurzübersicht für CKMT1B Antikörper (ABIN2855669)

Target: CKMT1B (Creatine Kinase, Mitochondrial 1B (CKMT1B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CKMT1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Produktdetails anti-CKMT1B Antikörper

Kreuzreaktivität: Human, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to Creatine kinase 1B (creatine kinase, mitochondrial 1B)
Creatine kinase 1B antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Creatine kinase MT. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: H1299 , HeLaS3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu CKMT1B

Target: CKMT1B (Creatine Kinase, Mitochondrial 1B (CKMT1B))

Andere Bezeichnung: creatine kinase, mitochondrial 1B

Hintergrund: Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80 % homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

Cellular Localization: Mitochondrion inner membrane, Peripheral membrane protein, Intermembrane side

Molekulargewicht: 47 kDa

Gen-ID: 1159

UniProt: P12532