Calretinin Antikörper (Calbindin 2) (Center)

Details for Product anti-CALB2 Antibody No. ABIN2855596, Anbieter: Anmelden zum Anzeigen
Antigen
  • CAB29
  • CAL2
  • CR
  • calb2l
  • wu:fq18e08
  • zgc:73115
  • calb2
  • wu:fq17g09
  • zgc:73099
  • calbindin 2
  • calbindin 2a
  • calbindin 2b
  • CALB2
  • Calb2
  • calb2a
  • calb2b
Epitop
Center
15
15
9
8
7
6
5
4
3
3
2
2
2
2
1
1
1
1
1
Reaktivität
Hund, Human, Maus
145
79
76
12
7
5
4
4
4
3
2
2
2
2
1
Wirt
Kaninchen
111
41
13
4
1
Klonalität
Polyklonal
Konjugat
Dieser Calretinin Antikörper ist unkonjugiert
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
116
76
51
47
23
13
13
11
11
10
7
6
6
2
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Antigen Calretinin
Chargennummer 39876
Validierte Anwendung Immunohistochemistry
Positivkontrolle

Adult (24 months) mouse and dog brain

Negativkontrolle

We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

Bewertung

ABIN2855596 works in IHC-P with microwave antigen retrieval pretreatment at an optimal concentration of 1:200.

Primärantikörper ABIN2855596
Sekundärantikörper goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
Protokoll
  • Obtain dog samples after autopsy and immersion-fix in buffered formaldehyde for 16h.
  • Perfuse mice with paraformaldehyde 4% in 0.1M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2h at RT.
  • Wash, dehydrate, and embed samples in paraffin wax.
  • Cut with a microtome into 6µm sections and mount on glass slides.
  • After paraffin removal, process sections by microwave antigen retrieval for 10min (95-100°C) in 10mM sodium citrate buffer (pH 6.0).
  • Let sections cool for 10min.
  • Wash sections under running water for 5-10min.
  • Wash sections 2x 5min with distilled water.
  • Wash sections 5min with 0.01M PBS.
  • Incubate sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) for 1h at RT to block non-specific binding sites.
  • Incubate sections with primary rabbit anti-Calretinin antibody (antibodies-online, ABIN2855596, lot 39876) diluted 1:200 in PBS-BSA-PLL at 4°C.
  • Wash sections 3x 5min with 0.01M PBS.
  • Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1M PBS for 1h at RT.
  • Wash sections 3x 5min with 0.01M PBS.
  • Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
  • Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with the manufacturer’s FITC filter set and digital camera at magnifications of 20x, 40x, and 63x. Parameters for image acquisition (Exposure 2.2s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.
Anmerkungen

For the incubation with the primary antibody ABIN2669973 1:100, 1:500, and 1:1000 dilutions were also tested.

Validierungsbilder
Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) Staining of dog and mouse brain sections using ABIN2855596. A-C: Specific staining of...
Immunogen Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
Isotyp IgG
Kreuzreaktivität Huhn, Schwein, Ratte (Rattus), Rind (Kuh)
Kreuzreaktivität (Details) Chicken (89 %), Pig (96 %), Rat (98 %), Bovine (97 %)
Produktmerkmale Rabbit Polyclonal antibody to Calretinin (calbindin 2)
Calretinin antibody
Reinigung Purified by antigen-affinity chromatography.
Plasmids, Primers & others Plasmide, Primers & weitere Calretinin products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Calretinin (CALB2 Antibody Abstract)
Hintergrund This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described.

Molekulargewicht 32 kDa
Gen-ID 794
Applikations-hinweise Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Kommentare

Positive Control: A431 , mouse brain , human CALB2-transfected 293T cells

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.75 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungs-mittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calret...
Western Blotting (WB) image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore b...
Western Blotting (WB) image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) WB Image Calretinin antibody detects CALB2 protein by Western blot analysis. A. 30 ug...
Immunohistochemistry (IHC) image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Cal...
Western Blotting (WB) image for anti-Calbindin 2 (CALB2) (Center) antibody (ABIN2855596) WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibo...
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