BIN1 Antikörper

Produktnummer ABIN2855595

Dieses Anti-BIN1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von BIN1 in WB, IHC, IF, ICC und IHC (p). Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für BIN1 Antikörper (ABIN2855595)

Target: BIN1 (Bridging Integrator 1 (BIN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BIN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BIN1 Antikörper

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to BIN1 (bridging integrator 1)
BIN1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BIN1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Validation: Comparison

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.35 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-BIN1 Antikörper (ABIN2855595)

Chan Wah Hak, Khan, Di Meglio, Law, Lucken-Ardjomande Häsler, Quintaneiro, Ferreira, Krause, McMahon, Boucrot: "FBP17 and CIP4 recruit SHIP2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis." in: Nature cell biology, Vol. 20, Issue 9, pp. 1023-1031, (2019) (PubMed).

Details zu BIN1

Target: BIN1 (Bridging Integrator 1 (BIN1))

Andere Bezeichnung: bridging integrator 1

Hintergrund: This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Cellular Localization: Isoform BIN1: Nucleus , Isoform IIA: Cytoplasm

Molekulargewicht: 65 kDa

Gen-ID: 274

UniProt: O00499