ACTN1 Antikörper

Produktnummer ABIN2855589

Produktdetails anti-ACTN1 Antikörper

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACTN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Kreuzreaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to alpha Actinin 1 (actinin, alpha 1)
alpha Actinin 1 antibody [N2N3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: NIH-3T3 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.09 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Andere Bezeichnung: actinin alpha 1 (ACTN1 Produkte)

Hintergrund: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk

Molekulargewicht: 103 kDa

Gen-ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization