ACTN2 Antikörper

Produktnummer ABIN2855582

Der Kaninchen Polyklonal Anti-ACTN2-Antikörper wurde für WB, IHC, IF, ICC und IHC (p) validiert. Er ist geeignet, ACTN2 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für ACTN2 Antikörper (ABIN2855582)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACTN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ACTN2 Antikörper

Spezifität: Based on sequence homology, this antibody may cross-react with other Actinin family members.

Kreuzreaktivität: Human, Maus, Schwein, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to alpha Actinin 2 (actinin, alpha 2)
alpha Actinin 2 antibody [N1N3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.18 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ACTN2 Antikörper (ABIN2855582)

Roberts, Romero, Mobley, Mumford, Roberson, Haun, Vann, Osburn, Holmes, Greer, Lockwood, Parry, Kavazis: "Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training." in: PeerJ, Vol. 6, pp. e5338, (2018) (PubMed).

Details zu ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Andere Bezeichnung: actinin alpha 2

Hintergrund: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

Cellular Localization: Cytoplasm , myofibril , sarcomere , Z-disk

Molekulargewicht: 104 kDa

Gen-ID: 88

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity