SLC32A1 Antikörper (N-Term)

Produktnummer ABIN2855225

Produktdetails anti-SLC32A1 Antikörper

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC32A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Multiplex Immunohistochemistry (mIHC)

Produktmerkmale: Rabbit Polyclonal antibody to VGAT (solute carrier family 32 (GABA vesicular transporter), member 1)
VGAT antibody [N1N2], N-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: U87-MG , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (mIHC)

Validierung #104339 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104339

Datum: 20.04.2021

Antigen: SLC32A1

Chargennummer: 42838

Validierte Anwendung: Multiplex Immunohistochemistry

Positivkontrolle:

FFPE normal human cortex

Negativkontrolle:

Unlabeled control (FFPE normal human cortex)

Bewertung:

Passed. The anti-SLC32A1 antibody ABIN2855225 produces punctate staining in close approximation with dendritic processes. This pattern is consistent with presynaptic staining, which is the expected site of SLC31A1 immunofluorescence.

Validierungsbilder

FFPE normal human cortex tissue section labeled with anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SYN1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488).

Protokoll

Primärantikörper: ABIN2855225

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SLC32A1 antibody ABIN2855225 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Anmerkungen:

• No signal was detected in unlabeled specimens.

• Specific staining of SLC32A1 was observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu SLC32A1

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Andere Bezeichnung: VGAT (SLC32A1 Produkte)

Synonyme: VGAT antikoerper, VIAAT antikoerper, vGAT antikoerper, Ci-vGAT antikoerper, slc32a1 antikoerper, viaat antikoerper, MGC68938 antikoerper, vgat antikoerper, xVIAAT antikoerper, R75019 antikoerper, Viaat antikoerper, CG8394 antikoerper, CG8394.2 antikoerper, Dmel\\CG8394 antikoerper, Vgat antikoerper, zgc:158324 antikoerper, solute carrier family 32 member 1 antikoerper, vesicular GABA transporter antikoerper, solute carrier family 32 member 1 S homeolog antikoerper, vesicular inhibitory amino acid transporter antikoerper, Vesicular inhibitory amino acid transporter antikoerper, solute carrier family 32 (GABA vesicular transporter), member 1 antikoerper, Vesicular GABA Transporter antikoerper, SLC32A1 antikoerper, vgat antikoerper, Bm1_20950 antikoerper, slc32a1.S antikoerper, slc32a1 antikoerper, CpipJ_CPIJ002704 antikoerper, viaat antikoerper, Slc32a1 antikoerper, VGAT antikoerper, Tsp_04599 antikoerper

Hintergrund: The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.

Cellular Localization: Cytoplasmic vesicle membrane, Multi-pass membrane protein

Molekulargewicht: 57 kDa

Gen-ID: 140679

Pathways: Proton Transport