Actin, gamma 1 Antikörper (N-Term)

Produktnummer ABIN2855174

Der Kaninchen Polyklonal Anti-Actin, gamma 1-Antikörper wurde für WB, IHC (p) und IHC (fro) validiert. Er ist geeignet, Actin, gamma 1 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Actin, gamma 1 Antikörper (N-Term) (ABIN2855174)

Target: Actin, gamma 1 (ACTG1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin, gamma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Actin, gamma 1 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Cat, Hund, Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)
alpha Sarcomeric Actin antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-Actin, gamma 1 Antikörper (ABIN2855174)

Xie, Perrino: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." in: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).

Chen, Wang, Wei, Tzeng, Lan, Cheng, Kuo: "Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer." in: Cancers, Vol. 11, Issue 8, (2019) (PubMed).

Li, Sasse, Bayguinov, Ward, Perrino: "Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes." in: Journal of diabetes research, Vol. 2018, pp. 8743874, (2018) (PubMed).

Details zu Actin, gamma 1

Target: Actin, gamma 1 (ACTG1)

Andere Bezeichnung: actin gamma 1

Hintergrund: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Cellular Localization: Cytoplasm , cytoskeleton

Molekulargewicht: 42 kDa

Gen-ID: 71

UniProt: P63261

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization