PAX8 Antikörper

Produktnummer ABIN2855097

Dieses Anti-PAX8-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PAX8 in WB, IHC, IF, IHC (p), IP, ICC und ChIP. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für PAX8 Antikörper (ABIN2855097)

Target: PAX8 (Paired Box 8 (PAX8))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Güteklasse: KO Validated

Produktdetails anti-PAX8 Antikörper

Kreuzreaktivität: Hund, Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to PAX8 (paired box 8)
PAX8 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T nuclear extract , DDDDK-tagged PAX8-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.87 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-PAX8 Antikörper (ABIN2855097)

Shi, Yin, Cai, Yan, Jia, Ma, Zhang, Zhang, Gu, Zhang, Di, Zhuang: "PAX8 regulon in human ovarian cancer links lineage dependency with epigenetic vulnerability to HDAC inhibitors." in: eLife, Vol. 8, (2020) (PubMed).

Sun, Lu, Wu, Ouyang, Xing, Hou, Shi, Wu: "PTEN-knockdown disrupts the morphology, growth pattern and function of Nthy-Ori 3-1 cells by downregulating PAX8 expression." in: Oncology letters, Vol. 18, Issue 6, pp. 6732-6740, (2019) (PubMed).

Details zu PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Andere Bezeichnung: paired box 8

Hintergrund: This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described.

Cellular Localization: Nucleus

Molekulargewicht: 48 kDa

Gen-ID: 7849

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation