Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

OCT4 Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch OCT4 in WB, IF, ICC, IP, FACS, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.
Produktnummer ABIN2855068

Kurzübersicht für OCT4 Antikörper (ABIN2855068)

Target

Alle OCT4 (POU5F1) Antikörper anzeigen
OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität

  • 142
  • 67
  • 32
  • 7
  • 7
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 120
  • 38
  • 3
  • 1
Kaninchen

Klonalität

  • 104
  • 56
  • 1
Polyklonal

Konjugat

  • 118
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser OCT4 Antikörper ist unkonjugiert

Applikation

  • 123
  • 60
  • 40
  • 31
  • 28
  • 26
  • 22
  • 15
  • 15
  • 14
  • 8
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Güteklasse

KO Validated
  • Kreuzreaktivität

    Human, Maus, Ratte

    Produktmerkmale

    Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)
    Oct4 antibody

    Aufreinigung

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

    Isotyp

    IgG
  • Applikationshinweise

    WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Kommentare

    Positive Control: mouse ESC , human ESC , NT2D1

    Validation: KO/KD, Orthogonal, Overexpression

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.49 mg/mL

    Buffer

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Feng, Su, Lin, Lin, Wu, Tung, Li, Shieh, Shiau: "Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer." in: BMC cancer, Vol. 19, Issue 1, pp. 791, (2020) (PubMed).

    Lee, Liu, Chang, Nieh, Lin, Jao, Chen, Liu: "Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative." in: Oncotarget, Vol. 7, Issue 17, pp. 23512-20, (2018) (PubMed).

    Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

    Chen, Huang, Lin: "Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma." in: International journal of oral science, Vol. 6, Issue 4, pp. 212-8, (2015) (PubMed).

  • Target

    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

    Andere Bezeichnung

    POU class 5 homeobox 1

    Hintergrund

    This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

    Cellular Localization: Nucleus

    Molekulargewicht

    39 kDa

    Gen-ID

    5460

    UniProt

    Q01860

    Pathways

    Stem Cell Maintenance
Sie sind hier:
Chat with us!