COX5A Antikörper (Center)

Produktnummer ABIN2855063

Der Kaninchen-Polyklonal Anti-COX5A-Antikörper wurde für WB, IHC (p), IF und ICC validiert. Er ist geeignet zum Nachweis von COX5A in Proben aus Human und Maus. }

Kurzübersicht für COX5A Antikörper (Center) (ABIN2855063)

Target: COX5A (Cytochrome C Oxidase Subunit Va (COX5A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COX5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-COX5A Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Affe, Ratte (Rattus), Rind (Kuh)

Kreuzreaktivität (Details): Monkey (97 %), Rat (93 %), Bovine (93 %)

Produktmerkmale: Rabbit Polyclonal antibody to COX5A (cytochrome c oxidase subunit Va)
COX5A antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu COX5A

Target: COX5A (Cytochrome C Oxidase Subunit Va (COX5A))

Andere Bezeichnung: COX5A

Hintergrund: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.

Cellular Localization: Mitochondrion inner membrane

Molekulargewicht: 17 kDa

Gen-ID: 9377

Pathways: Proton Transport