ALDOB Antikörper

Produktnummer ABIN2855024

Der Kaninchen Polyklonal Anti-ALDOB-Antikörper wurde für WB, IF, IHC (p) und ICC validiert. Er ist geeignet, ALDOB in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für ALDOB Antikörper (ABIN2855024)

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALDOB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-ALDOB Antikörper

Kreuzreaktivität: Human, Maus, Ratte, Schildkröte

Produktmerkmale: Rabbit Polyclonal antibody to Aldolase B (aldolase B, fructose-bisphosphate)
Aldolase B antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro2A , GL261 , C8D30 , PC-12 , mouse brain , rat brain , 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.09 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ALDOB Antikörper (ABIN2855024)

Chen, Lin, Chen, Cheng, Cheng, Imai et al.: "Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).

Dawson, Biggar, Storey: "Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure." in: PLoS ONE, Vol. 8, Issue 7, pp. e68830, (2014) (PubMed).

Details zu ALDOB

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Andere Bezeichnung: aldolase, fructose-bisphosphate B

Hintergrund: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5 % of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Molekulargewicht: 39 kDa

Gen-ID: 229

UniProt: P05062