ALPL Antikörper (Center)

Produktnummer ABIN2854870

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ALPL in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Schaf und wurde in 1 Publikation erwähnt.

Kurzübersicht für ALPL Antikörper (Center) (ABIN2854870)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reaktivität: Human, Maus, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALPL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ALPL Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Maus, Katze, Hund, Ratte (Rattus), Rind (Kuh)

Kreuzreaktivität (Details): Mouse (91 %), Cat (90 %), Dog (89 %), Rat (91 %), Bovine (89 %)

Produktmerkmale: Rabbit polyclonal antibody to alkaline phosphatase (liver/bone/kidney) (alkaline phosphatase, liver/bone/kidney)
Alkaline Phosphatase (Tissue Non-Specific) antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: HeLa , HepG2 , Molt-4 , Raji

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.48 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ALPL Antikörper (ABIN2854870)

Agustina, Asyifa, Aziz, Hernowo: "The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis." in: Pathology research international, Vol. 2018, pp. 6346409, (2018) (PubMed).

Details zu ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Andere Bezeichnung: Alkaline Phosphatase (Liver/bone/kidney)

Hintergrund: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Cellular Localization: Cell membrane, Lipid-anchor , GPI-anchor

Molekulargewicht: 57 kDa

Gen-ID: 249