ACSL4 Antikörper (C-Term)

Produktnummer ABIN2854678

Produktdetails anti-ACSL4 Antikörper

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACSL4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to FACL4 (acyl-CoA synthetase long-chain family member 4)
FACL4 antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:1000-1:5000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.44 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ACSL4 Antikörper (ABIN2854678)

Ndiaye, Liu, Hall, Minogue, Morgan, Waugh: "Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases." in: Bioscience reports, Vol. 40, Issue 4, (2020) (PubMed).

Garcia-Bermudez, Baudrier, Bayraktar, Shen, La, Guarecuco, Yucel, Fiore, Tavora, Freinkman, Chan, Lewis, Min, Inghirami, Sabatini, Birsoy: "Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death." in: Nature, Vol. 567, Issue 7746, pp. 118-122, (2019) (PubMed).

Radif, Ndiaye, Kalantzi, Jacobs, Hall, Minogue, Waugh: "The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells." in: Molecular and cellular biochemistry, Vol. 448, Issue 1-2, pp. 275-286, (2018) (PubMed).

Details zu ACSL4

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Andere Bezeichnung: acyl-CoA synthetase long chain family member 4 (ACSL4 Produkte)

Hintergrund: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

Cellular Localization: Mitochondrion outer membrane, Single-pass type III membrane protein , Peroxisome membrane, Single-pass type III membrane protein , Microsome membrane, Single-pass type III membrane protein , Endoplasmic reticulum membrane, Single-pass type III membrane pr

Molekulargewicht: 79 kDa

Gen-ID: 2182

UniProt: O60488