NOTCH2 Antikörper (C-Term)

Produktnummer ABIN2854343

Der Kaninchen Polyklonal Anti-NOTCH2-Antikörper wurde für WB und ChIP-seq validiert. Er ist geeignet, NOTCH2 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für NOTCH2 Antikörper (C-Term) (ABIN2854343)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTCH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-NOTCH2 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Rat (89 %)

Produktmerkmale: Rabbit polyclonal antibody to NOTCH2 (Notch homolog 2 (Drosophila))
NOTCH2 antibody [C2], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human NOTCH2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference Western blot 1:500-1:3000* ChIP-sequencing Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000* ChIP-sequencingAssay-dependent dilution

Kommentare:

Positive Control: A431 , HeLa , HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-NOTCH2 Antikörper (ABIN2854343)

Lin, Xie, Lin, Fu, Su, Tong, Chen, Wang, Zhao, Xia, Zhang, Zhang: "Epistatic Association of CD14 and NOTCH2 Genetic Polymorphisms with Biliary Atresia in a Southern Chinese Population." in: Molecular therapy. Nucleic acids, Vol. 13, pp. 590-595, (2018) (PubMed).

Details zu NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: NOTCH2

Hintergrund: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Notch 2 intracellular domain: Nucleus

Molekulargewicht: 265 kDa

Gen-ID: 4853

Pathways: Notch Signalweg, Stem Cell Maintenance