NPT II Antikörper

Produktnummer ABIN285052

Produktdetails

Target: NPT II

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Immunogen: MPTII antibody was raised in rabbit using purified neomycin phosphotransferase II as the immunogen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Use at starting dilution 1:500
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: Supplied as an IgG fraction in PBS, pH 7.4 with 0.05 % NaN3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -80 °C for long term storage.

Referenzen

Fu, Guo, Alfano: "Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin." in: Journal of bacteriology, Vol. 188, Issue 17, pp. 6060-9, (2006) (PubMed).

Antigendetails

Target: NPT II

Andere Bezeichnung: NPTII

Hintergrund: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.