MAP4K1 Antikörper (pSer171)

Produktnummer ABIN2794221

Dieses Anti-MAP4K1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MAP4K1 in DB. Geeignet für Human.

Kurzübersicht für MAP4K1 Antikörper (pSer171) (ABIN2794221)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP4K1 Antikörper ist unkonjugiert

Applikation: Dot Blot (DB)

Produktdetails anti-MAP4K1 Antikörper

Bindungsspezifität: pSer171

Verwendungszweck: Rabbit Anti-Human Phospho-MAP4K1(S171) Antibody

Immunogen: This MAP4K1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S171 of human MAP4K1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Dot Blot
Recommended Dilutions
DB: 1:500
Dot blot analysis of anti-MAP4K1-pS171 Phospho-specific Pab (RB12751) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 μg per ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.340 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C (short-term), -20°C (long-term)

Details zu MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Andere Bezeichnung: MAP4K1

Hintergrund:

Target Description: The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.

Gene Symbol: MAP4K1

Molekulargewicht: 91296 Da

Gen-ID: 11184

UniProt: Q92918

Pathways: T-Zell Rezeptor Signalweg, Signaling of Hepatocyte Growth Factor Receptor