OCT4 Antikörper (N-Term)
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- Target Alle OCT4 (POU5F1) Antikörper anzeigen
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Bindungsspezifität
- N-Term
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Reaktivität
- Human, Schwein, Hund, Kaninchen, Ziege
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser OCT4 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Sequenz
- PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT
- Kreuzreaktivität
- Rind (Kuh), Hund, Human, Schwein, Kaninchen
- Homologie
- Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%
- Produktmerkmale
- This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.
- Aufreinigung
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
- Top Product
- Discover our top product POU5F1 Primärantikörper
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- Applikationshinweise
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WB Suggested Anti-POU5F1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. - Kommentare
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Antigen size: 360 AA
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).
: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." in: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).
: "Oct4 pseudogenes are transcribed in cancers." in: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).
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BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).
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- Target
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Andere Bezeichnung
- OCT3/4 (POU5F1 Produkte)
- Synonyme
- OCT3 antikoerper, OCT4 antikoerper, OTF-3 antikoerper, OTF3 antikoerper, OTF4 antikoerper, Oct-3 antikoerper, Oct-4 antikoerper, POU5F1 antikoerper, gp9 antikoerper, gp-9 antikoerper, oct4 antikoerper, pou2 antikoerper, POU-2 antikoerper, cb197 antikoerper, spg/pou2 antikoerper, chunp6868 antikoerper, wu:fd18d06 antikoerper, NF-A3 antikoerper, Oct-3/4 antikoerper, Oct3 antikoerper, Oct3/4 antikoerper, Oct4 antikoerper, Otf-3 antikoerper, Otf-4 antikoerper, Otf3 antikoerper, Otf3-rs7 antikoerper, Otf3g antikoerper, Otf4 antikoerper, POU class 5 homeobox 1 antikoerper, POU domain, class 5, transcription factor 3 antikoerper, POU domain, class 5, transcription factor 1 antikoerper, POU5F1 antikoerper, pou5f3 antikoerper, Pou5f1 antikoerper
- Hintergrund
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POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
Protein Size: 360 - Molekulargewicht
- 39 kDa
- Gen-ID
- 5460
- NCBI Accession
- NM_002701, NP_002692
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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