E2F5 Antikörper (N-Term)

Produktnummer ABIN2792655

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch E2F5 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen und Zebrafisch (Danio rerio).

Kurzübersicht für E2F5 Antikörper (N-Term) (ABIN2792655)

Target: E2F5

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-E2F5 Antikörper

Bindungsspezifität: N-Term

Sequenz: KAEIEDLELK ERELDQQKLL LQQSIKNVMD DSINNRFSYV THEDICNCFN

Kreuzreaktivität: Huhn, Human, Maus, Ratte (Rattus)

Homologie: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 84%

Produktmerkmale: This is a rabbit polyclonal antibody against E2F5. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F5

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 346 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu E2F5

Target: E2F5

Andere Bezeichnung: E2F5

Hintergrund: E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
Alias Symbols: E2F-5
Protein Interaction Partner: RBL2, UBC, KMT2D, BRCC3, SMAD3, EP300, XPO1, CREBBP, TFDP1, RBL1, BIRC5, MYC, MT1G,
Protein Size: 346

Molekulargewicht: 38 kDa

Gen-ID: 1875

NCBI Accession: NM_001951, NP_001942

UniProt: Q15329