PRDM1 Antikörper (Middle Region)

Produktnummer ABIN2792524

Dieser Anti-PRDM1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von PRDM1 in WB und IHC. Geeignet für Human, Maus, Ratte, Hund, Schwein, Rind (Kuh), Pferd, Kaninchen und Meerschweinchen. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für PRDM1 Antikörper (Middle Region) (ABIN2792524)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRDM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-PRDM1 Antikörper

Bindungsspezifität: Middle Region

Sequenz: VEDDISVISV VEKEILAVVR KEKEETGLKV SLQRNMGNGL LSSGCSLYES

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte (Rattus)

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 84%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 92%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against PRDM1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRDM1

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-PRDM1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: SH-SYSY cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 691 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-PRDM1 Antikörper (ABIN2792524)

Arulanandam, Batenchuk, Angarita, Ottolino-Perry, Cousineau, Mottashed, Burgess, Falls, De Silva, Tsang, Howe, Bourgeois-Daigneault, Conrad, Daneshmand, Breitbach, Kirn, Raptis, Sad, Atkins, Huh et al.: "VEGF-Mediated Induction of PRD1-BF1/Blimp1 Expression Sensitizes Tumor Vasculature to Oncolytic Virus Infection. ..." in: Cancer cell, Vol. 28, Issue 2, pp. 210-24, (2015) (PubMed).

Details zu PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Andere Bezeichnung: PRDM1 / BLIMP1

Hintergrund: PRDM1 is a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Alias Symbols: BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1
Protein Interaction Partner: PRMT5, SENP1, EHMT2, PIAS1, SUMO1, IRF2, IRF1, HDAC2, HDAC1, HIST1H1A, MS4A1, HSP90AA1, ATXN1, ELAVL1, KDM1A, IRF4, TLE2, TLE1, AES, PRMT7, HIST2H3A,
Protein Size: 691

Molekulargewicht: 77 kDa

Gen-ID: 639

NCBI Accession: NM_182907, NP_878911

UniProt: Q86WM7

Pathways: Regulation of Muscle Cell Differentiation