NAPA Antikörper (N-Term)

Produktnummer ABIN184245

Der Kaninchen Polyklonal anti-NAPA Antikörper (ABIN184245) detektiert spezifisch NAPA in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Maus, Rind (Kuh), Hund und Zebrafisch (Danio rerio).

Kurzübersicht für NAPA Antikörper (N-Term) (ABIN184245)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reaktivität: Maus, Rind (Kuh), Hund, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NAPA Antikörper

Bindungsspezifität: N-Term

Sequenz: MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN

Homologie: Cow: 100%, Dog: 100%, Mouse: 78%, Zebrafish: 78%

Produktmerkmale: This is a rabbit polyclonal antibody against NAPA. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NAPA

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 295 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Andere Bezeichnung: NAPA

Hintergrund: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively.
Alias Symbols: SNAPA
Protein Interaction Partner: UBC, RPA3, RPA2, RPA1, RNF2, ATP4A, STX5, ITGA4, FN1, VCAM1, VCP, DHX40, DHX29, THUMPD3, STAM2, VAMP2, APP, NSF, TRPC3, RTN4, STX12, GNA12, STX8, GOSR2, GOSR1, BNIP1, STX7, VAMP8, SNAP23, STX4, STX1A, SNAP25,
Protein Size: 295

Molekulargewicht: 33 kDa

Gen-ID: 8775

NCBI Accession: NM_003827, NP_003818

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization