NAE1 Antikörper (C-Term)

Produktnummer ABIN2788045

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NAE1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Schwein, Kaninchen und Zebrafisch (Danio rerio).

Kurzübersicht für NAE1 Antikörper (C-Term) (ABIN2788045)

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Schwein, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NAE1 Antikörper

Bindungsspezifität: C-Term

Sequenz: ARALKEFVAK EGQGNLPVRG TIPDMIADSN KYIKLQNVYR EKAKKDAAAV

Homologie: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against Nae1. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Nae1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 534 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu NAE1

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Andere Bezeichnung: Nae1

Hintergrund: Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Alias Symbols: 59 kDa, Appbp1
Protein Size: 534

Molekulargewicht: 60 kDa

Gen-ID: 234664

NCBI Accession: NM_144931, NP_659180

UniProt: Q8VBW6