NARF Antikörper (Middle Region)

Produktnummer ABIN2785739

Der Kaninchen Polyklonal anti-NARF Antikörper wird verwendet zum Nachweis von NARF in Proben von Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund und Meerschweinchen. Er wurde validiert für WB.

Kurzübersicht für NARF Antikörper (Middle Region) (ABIN2785739)

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reaktivität: Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NARF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NARF Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: NARF Antibody - middle region

Sequenz: FRNIQNMILK LKKGKFPFHF VEVLACAGGC LNGRGQAQTP DGHADKALLR

Homologie: Cow: 92%, Dog: 86%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 79%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against NARF. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NARF

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54490-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu NARF

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Andere Bezeichnung: NARF

Hintergrund:

Background Information: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

Gene Name: Nuclear prelamin A recognition factor

Alternative Symbols: IOP2

Protein Name: Nuclear prelamin A recognition factor

Molekulargewicht: 55kDa

Gen-ID: 26502

NCBI Accession: NP_114174

UniProt: Q9UHQ1