NARF Antikörper (Middle Region)

Produktnummer ABIN2785739

Der Kaninchen-Polyklonal Anti-NARF-Antikörper wurde für WB validiert. Er ist geeignet zum Nachweis von NARF in Proben aus Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund und Meerschweinchen. }

Kurzübersicht für NARF Antikörper (Middle Region) (ABIN2785739)

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reaktivität: Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NARF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NARF Antikörper

Bindungsspezifität: Middle Region

Sequenz: FRNIQNMILK LKKGKFPFHF VEVLACAGGC LNGRGQAQTP DGHADKALLR

Homologie: Cow: 92%, Dog: 86%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 79%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against NARF. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NARF

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 502 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu NARF

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Andere Bezeichnung: NARF

Hintergrund: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Alias Symbols: DKFZp434G0420, FLJ10067, IOP2
Protein Interaction Partner: UBC, LEF1, STAT5A, SHFM1, MMS19, CBX5, APP, LMNA,
Protein Size: 502

Molekulargewicht: 55 kDa

Gen-ID: 26502

NCBI Accession: NM_031968, NP_114174