CCDC42 Antikörper (Middle Region)

Produktnummer ABIN2785068

Zitiert in 2+ Publikationen. Dieser Anti-CCDC42-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von CCDC42 in WB. Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen. Unabhängig validiert für die Anwendung in Immunocytochemistry.

Kurzübersicht für CCDC42 Antikörper (Middle Region) (ABIN2785068)

Target: CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCDC42 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CCDC42 Antikörper

Bindungsspezifität: Middle Region

Sequenz: ENSEFEEIHE VIARYKTLVS MHHDLMQSAQ EGQEKIEHAK ARLARYMEEK

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against Ccdc42. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Rat Ccdc42

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 316 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (ICC)

Validierung #103398 (Immunocytochemistry)

by: Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen

No.: #103398

Datum: 12.10.2018

Antigen: CCDC42

Chargennummer: QC28887-41199

Validierte Anwendung: Immunocytochemistry

Positivkontrolle: NIH/3T3 cells transfected with Cddc42-mCherry expression vector

Negativkontrolle: Non-transfected NIH/3T3 cells

Bewertung:

Passed. ABIN2785068 specifically detects Ccdc42-Cherry when exogenously expressed. Background staining is low.

Validierungsbilder

Cells were transfected with Ccdc42 tagged with mCherry (red) and incubated with ABIN2785068 (green). The pictures on the right show the red and green channels merged with DAPI counterstain (blue).

Protokoll

Primärantikörper: ABIN2785068

Sekundärantikörper: goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058)

Full Protocol:

• Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO₂ to 70% confluency.
• Transfect cells with Ccdc42 tagged mCherry (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
• Grow cells for 24h.
• Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
• Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
• Incubate cells with primary rabbit anti-Ccdc42 antibody (antibodies-online, ABIN2785068, QC28887-41199) diluted 1:100 in PBS for 1h at 37°C.
• Wash cells 3x 5min with 1x PBS.
• Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058) diluted 1:300 for 1h at 37°C in PBS.
• DNA was counterstained with DAPI (4’, 6-Diamidino-2-phenylindole; Sigma, D-9542).
• Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-CCDC42 Antikörper (ABIN2785068)

Wu, Yu, Liu, Wang, Huang, Lu, Chen, Li, Liu: "Essential Role of CFAP53 in Sperm Flagellum Biogenesis." in: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).

Tapia Contreras, Hoyer-Fender: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." in: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).

Details zu CCDC42

Target: CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))

Andere Bezeichnung: Ccdc42

Hintergrund: The function of this protein remains unknown.
Alias Symbols: RGD1565925
Protein Size: 316

Molekulargewicht: 34 kDa

Gen-ID: 303234

NCBI Accession: NM_001107009, NP_001100479

UniProt: D3ZAZ8