RFT1 Antikörper (N-Term)

Produktnummer ABIN2784093

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RFT1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Hund, Maus, Rind (Kuh), Meerschweinchen, Pferd und Kaninchen.

Kurzübersicht für RFT1 Antikörper (N-Term) (ABIN2784093)

Target: RFT1 (RFT1 Homolog (RFT1))

Reaktivität: Human, Ratte, Hund, Maus, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RFT1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: RFT1 Antibody - N-terminal region

Sequenz: GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV

Homologie: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFT1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110451

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu RFT1

Target: RFT1 (RFT1 Homolog (RFT1))

Andere Bezeichnung: RFT1

Hintergrund:

Background Information: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a ! !Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).[supplied by OMIM].

Gene Name: RFT1 homolog (S. cerevisiae)

Alternative Symbols: CDG1N

Protein Name: Protein RFT1 homolog

Molekulargewicht: 60kDa

Gen-ID: 91869

NCBI Accession: NP_443091

UniProt: Q96AA3