MANEA Antikörper (Middle Region)

Produktnummer ABIN2783932

Der Kaninchen Polyklonal anti-MANEA Antikörper wird verwendet zum Nachweis von MANEA in Proben von Human, Maus, Ratte, Hund, Pferd, Zebrafisch (Danio rerio), Schwein und Kaninchen. Er wurde validiert für WB.

Kurzübersicht für MANEA Antikörper (Middle Region) (ABIN2783932)

Target: MANEA (Mannosidase, Endo-alpha (MANEA))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Zebrafisch (Danio rerio), Schwein, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MANEA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MANEA Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: MANEA Antibody - middle region

Sequenz: KVTFHIEPYS NRDDQNMYKN VKYIIDKYGN HPAFYRYKTK TGNALPMFYV

Homologie: Dog: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 92%, Rat: 100%, Zebrafish: 77%

Produktmerkmale: This is a rabbit polyclonal antibody against MANEA. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MANEA

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP49845-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu MANEA

Target: MANEA (Mannosidase, Endo-alpha (MANEA))

Andere Bezeichnung: MANEA

Hintergrund:

Background Information: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans.

Gene Name: Mannosidase, endo-alpha

Alternative Symbols: ENDO, hEndo

Protein Name: Glycoprotein endo-alpha-1,2-mannosidase

Molekulargewicht: 54kDa

Gen-ID: 79694

NCBI Accession: NP_078917

UniProt: Q5SRI9