MANEA Antikörper (Middle Region)

Produktnummer ABIN2783932

Der Kaninchen Polyklonal Anti-MANEA-Antikörper wurde für WB validiert. Er ist geeignet, MANEA in Proben von Human, Maus, Ratte, Hund, Pferd, Zebrafisch (Danio rerio), Schwein und Kaninchen zu detektieren.

Kurzübersicht für MANEA Antikörper (Middle Region) (ABIN2783932)

Target: MANEA (Mannosidase, Endo-alpha (MANEA))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Zebrafisch (Danio rerio), Schwein, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MANEA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MANEA Antikörper

Bindungsspezifität: Middle Region

Sequenz: KVTFHIEPYS NRDDQNMYKN VKYIIDKYGN HPAFYRYKTK TGNALPMFYV

Homologie: Dog: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 92%, Rat: 100%, Zebrafish: 77%

Produktmerkmale: This is a rabbit polyclonal antibody against MANEA. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MANEA

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 462 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu MANEA

Target: MANEA (Mannosidase, Endo-alpha (MANEA))

Andere Bezeichnung: MANEA

Hintergrund: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans.
Alias Symbols: DKFZp686D20120, FLJ12838, hEndo, ENDO
Protein Interaction Partner: UBC, DNM2, PRD, AMPH,
Protein Size: 462

Molekulargewicht: 54 kDa

Gen-ID: 79694

NCBI Accession: NM_024641, NP_078917

UniProt: Q5SRI9