Atp5f1b (C-Term) Antikörper

Produktnummer ABIN2783269

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Atp5f1b in WB, IHC und IP. Es zeigt Reaktivität gegenüber Proben von Maus, Human, Ratte, Zebrafisch (Danio rerio), Saccharomyces cerevisiae, Kaninchen, Pferd, Meerschweinchen, Hund und Rind (Kuh). Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für Atp5f1b (C-Term) Antikörper (ABIN2783269)

Target: Atp5f1b

Reaktivität: Maus, Human, Ratte, Zebrafisch (Danio rerio), Saccharomyces cerevisiae, Kaninchen, Pferd, Meerschweinchen, Hund, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails

Bindungsspezifität: C-Term

Verwendungszweck: ATP5F1B Antibody - C-terminal region

Sequenz: MGKLVPLKET IKGFQQILAG EYDHLPEQAF YMVGPIEEAV AKADKLAEEH

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against ATP5B. It was validated on Western Blot using a cell lysate as a positive control. Catalog ABIN973616 can be used a blocking peptide for this product.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ATP5B

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48186-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen

Usui, Kajita, Kajita, Mori, Hanamoto, Ikeda, Okada, Taguchi, Kitada, Morita, Sasaki, Kitamura, Sato, Kojima, Ishizuka: "Elevated mitochondrial biogenesis in skeletal muscle is associated with testosterone-induced body weight loss in male mice." in: FEBS letters, Vol. 588, Issue 10, pp. 1935-41, (2014) (PubMed).

Rubel, Schisler, Hamlett, DeKroon, Gautel, Alzate, Patterson: "Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates." in: Cell biochemistry and biophysics, Vol. 67, Issue 1, pp. 127-38, (2013) (PubMed).

Teodoro, Duarte, Gomes, Varela, Peixoto, Rolo, Palmeira: "Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation." in: Mitochondrion, Vol. 13, Issue 6, pp. 637-46, (2013) (PubMed).

Antigendetails

Target: Atp5f1b

Hintergrund:

Targeted Subunit: beta, mitochondrial

Background Information: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Gene Name: ATP synthase F1 subunit beta

Alternative Symbols: ATP5B, ATPMB, ATPSB, HEL-S-271

Protein Name: ATP synthase subunit beta, mitochondrial

Molekulargewicht: 57 kDa

Gen-ID: 506

NCBI Accession: NP_001677

UniProt: P06576