CDC27 Antikörper (Middle Region)

Produktnummer ABIN2781488

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CDC27 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen und Meerschweinchen.

Kurzübersicht für CDC27 Antikörper (Middle Region) (ABIN2781488)

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reaktivität: Human, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CDC27 Antikörper

Bindungsspezifität: Middle Region

Sequenz: KLKMKFPPKI PNRKTKSKTN KGGITQPNIN DSLEITKLDS SIISEGKIST

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against CDC27. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CDC27

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 824 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Andere Bezeichnung: CDC27

Hintergrund: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: APC3, CDC27Hs, D0S1430E, D17S978E, HNUC, NUC2, ANAPC3
Protein Interaction Partner: ANAPC2, UBE2S, PTTG1, FBXO43, MAD2L1, COMT, CDC20, CCNB1, BUB1B, UBC, BIRC6, ANAPC4, CDH1, FZR1, MIB1, HECW2, ANAPC11, PHF8, CUEDC2, Sirt2, Dlg4, GKN1, ANAPC7, ANAPC5, DCPS, ADNP, MDC1, PTEN, TRIM33, REV1, ANAPC15, VCP, MAD2L2, NOTCH1, PAXIP1, UBE4B, TTK,
Protein Size: 824

Molekulargewicht: 92 kDa

Gen-ID: 996

NCBI Accession: NM_001256, NP_001247

UniProt: P30260