RAD17 Antikörper (C-Term)

Produktnummer ABIN2779300

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-RAD17 Antikörper (ABIN2779300) detektiert spezifisch RAD17 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Rind (Kuh) und Schwein.

Kurzübersicht für RAD17 Antikörper (C-Term) (ABIN2779300)

Target: RAD17

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAD17 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: RAD17 Antibody - C-terminal region

Sequenz: PTQATVPETW SLPLSQNSAS ELPASQPQPF SAQGDMEENI IIEDYESDGT

Homologie: Cow: 83%, Dog: 75%, Human: 100%, Mouse: 75%, Pig: 83%, Rat: 75%

Produktmerkmale: This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30206-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-RAD17 Antikörper (ABIN2779300)

Tsao, Geisen, Abraham: "Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling." in: The EMBO journal, Vol. 23, Issue 23, pp. 4660-9, (2004) (PubMed).

Details zu RAD17

Target: RAD17

Andere Bezeichnung: RAD17

Hintergrund:

Background Information: RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.

Gene Name: RAD17 homolog (S. pombe)

Alternative Symbols: CCYC, R24L, RAD24, HRAD17, RAD17SP

Protein Name: Cell cycle checkpoint protein RAD17

Molekulargewicht: 66kDa

Gen-ID: 5884

NCBI Accession: NP_579919

UniProt: O75943