CPSF4 Antikörper (C-Term)

Produktnummer ABIN2779005

Produktdetails anti-CPSF4 Antikörper

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Pferd, Rind (Kuh), Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPSF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: SLIQLTSQNS SPNQQRTPQV IGVMQSQNSS AGNRGPRPLE QVTCYKCGEK

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against CPSF4. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CPSF4

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 216 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu CPSF4

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Andere Bezeichnung: CPSF4 (CPSF4 Produkte)

Hintergrund: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.
Alias Symbols: CPSF30, NAR, NEB1
Protein Interaction Partner: FIP1L1, RBBP6, MEOX2, CLK2, NPM1, MDC1, ECT2, UBC, CSTF2T, RRAGA, CDC73, SLC12A9, MARK3,
Protein Size: 216

Molekulargewicht: 24 kDa

Gen-ID: 10898

NCBI Accession: NM_001081559

UniProt: O95639