SF3A1 Antikörper (N-Term)

Produktnummer ABIN2778958

Dieses Anti-SF3A1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SF3A1 in WB, IHC und IF. Geeignet für Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für SF3A1 Antikörper (N-Term) (ABIN2778958)

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SF3A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SF3A1 Antikörper

Bindungsspezifität: N-Term

Sequenz: QQTTQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against SF3A1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3A1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 793 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-SF3A1 Antikörper (ABIN2778958)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Details zu SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Andere Bezeichnung: SF3A1

Hintergrund: SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PRP21, PRPF21, SAP114, SF3A120
Protein Interaction Partner: HDAC1, FUS, HUWE1, UBC, RPA3, RPA2, RPA1, WWOX, ZBTB1, SUZ12, EED, RNF2, rev, NR3C1, APBB1, HNRNPR, GAS7, CCDC6, PPP4R2, BRCC3, SMEK1, PRPF40A, GAPVD1, WBP4, TCERG1, HDAC11, HDAC6, SF3A3, DDX42, RBM10, SF3A2, SF1, WHSC1, IL7R, CSNK2A1, WDR77, RBFOX2, SMUR
Protein Size: 793

Molekulargewicht: 89 kDa

Gen-ID: 10291

NCBI Accession: NM_005877, NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization