SF3A1 Antikörper (N-Term)

Produktnummer ABIN2778958

Dieser Anti-SF3A1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von SF3A1 in WB, IHC und IF. Geeignet für Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für SF3A1 Antikörper (N-Term) (ABIN2778958)

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SF3A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SF3A1 Antikörper

Bindungsspezifität: N-Term

Sequenz: QQTTQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against SF3A1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3A1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 793 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-SF3A1 Antikörper (ABIN2778958)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Details zu SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Andere Bezeichnung: SF3A1

Hintergrund: SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PRP21, PRPF21, SAP114, SF3A120
Protein Interaction Partner: HDAC1, FUS, HUWE1, UBC, RPA3, RPA2, RPA1, WWOX, ZBTB1, SUZ12, EED, RNF2, rev, NR3C1, APBB1, HNRNPR, GAS7, CCDC6, PPP4R2, BRCC3, SMEK1, PRPF40A, GAPVD1, WBP4, TCERG1, HDAC11, HDAC6, SF3A3, DDX42, RBM10, SF3A2, SF1, WHSC1, IL7R, CSNK2A1, WDR77, RBFOX2, SMUR
Protein Size: 793

Molekulargewicht: 89 kDa

Gen-ID: 10291

NCBI Accession: NM_005877, NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization