SMN1 Antikörper (N-Term)

Produktnummer ABIN2778671

Dieses Anti-SMN1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SMN1 in WB. Geeignet für Human, Maus, Ratte, Hund, Schwein, Kaninchen, Meerschweinchen und Pferd.

Kurzübersicht für SMN1 Antikörper (N-Term) (ABIN2778671)

Target: SMN1 (Survival of Motor Neuron 1, Telomeric (SMN1))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Kaninchen, Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SMN1 Antikörper

Bindungsspezifität: N-Term

Sequenz: KAVASFKHAL KNGDICETSG KPKTTPKRKP AKKNKSQKKN TAASLQQWKV

Homologie: Dog: 100%, Guinea Pig: 85%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 85%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against SMN1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMN1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 262 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu SMN1

Target: SMN1 (Survival of Motor Neuron 1, Telomeric (SMN1))

Andere Bezeichnung: SMN1

Hintergrund: SMN1 localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein.This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. However, mutations in this gene, the telomeric copy, are associated with spinal muscular atrophy, mutations in the centromeric copy do not lead to disease. The centromeric copy may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7 which is thought to be an exon splice enhancer. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein. Two transcript variants are produced by this gene.
Alias Symbols: BCD541, SMA, SMA1, SMA2, SMA3, SMA4, SMA@, SMN, SMNT, T-BCD541, SMN2
Protein Interaction Partner: COPA, RNF2, BMI1, KDM1A, SMN1, RN7SL1, UBC, HDAC11, MIB1, GEMIN4, DICER1, DDX20, PAN2, RBFOX2, HNRNPUL1, IQCB1, UBL4A, SMN2, NOS2, vpr, GEMIN2, SRP68, SRP54, SRP19, SRP9, RNU2-1, RNU1-1, GEMIN5, MDC1, RBM25, COIL, SRSF5, HSPB1, CUL3, DDAH2, MAST2, SMC5, R
Protein Size: 262

Molekulargewicht: 28 kDa

Gen-ID: 6606

NCBI Accession: NM_022874, NP_075012

UniProt: Q16637

Pathways: Ribonucleoprotein Complex Subunit Organization