PRMT2 Antikörper (N-Term)

Produktnummer ABIN2778662

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PRMT2 in WB und ChIP. Es zeigt Reaktivität gegenüber Proben von Human und Ratte. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für PRMT2 Antikörper (N-Term) (ABIN2778662)

Target: PRMT2 (Protein Arginine Methyltransferase 2 (PRMT2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRMT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-PRMT2 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: PRMT2 Antibody - N-terminal region

Sequenz: ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE EYFGSYGTLK LHLEMLADQP

Homologie: Human: 100%, Rat: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against PRMT2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40195-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-PRMT2 Antikörper (ABIN2778662)

Zhong, Cao, Liu, Liu, Wang, Liu, Chen, Yang, Zhang, Wu, Ding, Hong, Xiao, Zu, Wen: "Nuclear loss of protein arginine N-methyltransferase 2 in breast carcinoma is associated with tumor grade and overexpression of cyclin D1 protein." in: Oncogene, Vol. 33, Issue 48, pp. 5546-58, (2014) (PubMed).

Meyer, Wolf, Obendorf: "PRMT2, a member of the protein arginine methyltransferase family, is a coactivator of the androgen receptor." in: The Journal of steroid biochemistry and molecular biology, Vol. 107, Issue 1-2, pp. 1-14, (2007) (PubMed).

Details zu PRMT2

Target: PRMT2 (Protein Arginine Methyltransferase 2 (PRMT2))

Andere Bezeichnung: PRMT2

Hintergrund:

Background Information: The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.

Gene Name: Protein arginine methyltransferase 2

Alternative Symbols: HRMT1L1

Protein Name: Protein arginine N-methyltransferase 2

Molekulargewicht: 49kDa

Gen-ID: 3275

NCBI Accession: NP_001526

UniProt: P55345

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding