MBD3 Antikörper (N-Term)

Produktnummer ABIN2778641

Der Kaninchen Polyklonal anti-MBD3 Antikörper wird verwendet zum Nachweis von MBD3 in Proben von Human, Maus, Ratte, Hund, Meerschweinchen, Rind (Kuh) und Pferd. Er wurde validiert für WB.

Kurzübersicht für MBD3 Antikörper (N-Term) (ABIN2778641)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Rind (Kuh), Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MBD3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: MBD3 Antibody - N-terminal region

Sequenz: SKMNKSRQRV RYDSSNQVKG KPDLNTALPV RQTASIFKQP VTKITNHPSN

Homologie: Cow: 85%, Dog: 100%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MBD3. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD3

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40170-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Andere Bezeichnung: MBD3

Hintergrund:

Background Information: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Methyl-CpG binding domain protein 3

Alternative Symbols: -

Protein Name: Methyl-CpG-binding domain protein 3

Molekulargewicht: 33kDa

Gen-ID: 53615

NCBI Accession: NP_003917

UniProt: O95983

Pathways: Chromatin Binding