PHD1 Antikörper (Middle Region)

Produktnummer ABIN2778438

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-PHD1 Antikörper (ABIN2778438) detektiert spezifisch PHD1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Hund.

Kurzübersicht für PHD1 Antikörper (Middle Region) (ABIN2778438)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PHD1 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: EGLN2 Antibody - middle region

Sequenz: AVLDGSELSY FGQEGMTEVQ CGKVAFQFQC SSDSTNGTGV QGGQIPELIF

Homologie: Dog: 85%, Human: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against EGLN2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EGLN2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39775-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-PHD1 Antikörper (ABIN2778438)

Tennant, Frezza, MacKenzie, Nguyen, Zheng, Selak, Roberts, Dive, Watson, Aboagye, Gottlieb: "Reactivating HIF prolyl hydroxylases under hypoxia results in metabolic catastrophe and cell death." in: Oncogene, Vol. 28, Issue 45, pp. 4009-21, (2009) (PubMed).

Details zu PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Andere Bezeichnung: EGLN2

Hintergrund:

Background Information: The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms. The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene Name: Egl nine homolog 2 (C. elegans)

Alternative Symbols: EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1

Protein Name: Egl nine homolog 2

Molekulargewicht: 44kDa

Gen-ID: 112398

NCBI Accession: NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis