MED27 Antikörper (Middle Region)

Produktnummer ABIN2778099

Der Kaninchen Polyklonal anti-MED27 Antikörper (ABIN2778099) detektiert spezifisch MED27 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen.

Kurzübersicht für MED27 Antikörper (Middle Region) (ABIN2778099)

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MED27 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: MED27 Antibody - middle region

Sequenz: LSRPNGTSAM LLVTLGKVLK VIVVMRSLFI DRTIVKGYNE NVYTEDGKLD

Homologie: Cow: 100%, Dog: 85%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MED27. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED27

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33743-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Andere Bezeichnung: MED27

Hintergrund:

Targeted Subunit: 27

Background Information: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Gene Name: Mediator complex subunit 27

Alternative Symbols: MED3, CRSP8, CRAP34, CRSP34, TRAP37

Protein Name: Mediator of RNA polymerase II transcription subunit 27

Molekulargewicht: 35kDa

Gen-ID: 9442

NCBI Accession: NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha