MRPS15 Antikörper (N-Term)

Produktnummer ABIN2777848

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-MRPS15 Antikörper wird verwendet zum Nachweis von MRPS15 in Proben von Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd und Hund. Er wurde validiert für WB und IHC.

Kurzübersicht für MRPS15 Antikörper (N-Term) (ABIN2777848)

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MRPS15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-MRPS15 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: MRPS15 Antibody - N-terminal region

Sequenz: RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVVK RLLSLEMANK

Homologie: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 83%, Rabbit: 86%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against MRPS15. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPS15

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33299-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-MRPS15 Antikörper (ABIN2777848)

Davies, Rackham, Shearwood, Hamilton, Narsai, Whelan, Filipovska: "Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation." in: FEBS letters, Vol. 583, Issue 12, pp. 1853-8, (2009) (PubMed).

Details zu MRPS15

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Andere Bezeichnung: MRPS15

Hintergrund:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

Gene Name: Mitochondrial ribosomal protein S15

Alternative Symbols: DC37, S15mt, RPMS15, MPR-S15

Protein Name: 28S ribosomal protein S15, mitochondrial

Molekulargewicht: 30kDa

Gen-ID: 64960

NCBI Accession: NP_112570

UniProt: P82914