ERI1 Antikörper (N-Term)

Produktnummer ABIN2776656

Dieses Anti-ERI1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ERI1 in WB. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für ERI1 Antikörper (N-Term) (ABIN2776656)

Target: ERI1 (Exoribonuclease 1 (ERI1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ERI1 Antikörper

Bindungsspezifität: N-Term

Sequenz: SKFITSSASD FSDPVYKEIA ITNGCINRMS KEELRAKLSE FKLETRGVKD

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 85%, Pig: 93%, Rabbit: 100%, Rat: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against THEX1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERI1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 349 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-ERI1 Antikörper (ABIN2776656)

Kupsco, Wu, Marzluff, Thapar, Duronio: "Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleases." in: RNA (New York, N.Y.), Vol. 12, Issue 12, pp. 2103-17, (2006) (PubMed).

Details zu ERI1

Target: ERI1 (Exoribonuclease 1 (ERI1))

Andere Bezeichnung: THEX1

Hintergrund: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Alias Symbols: HEXO, THEX1, 3'HEXO
Protein Interaction Partner: UBC, HNRNPD, HHV8GK18_gp81, SLBP,
Protein Size: 38

Molekulargewicht: 349 kDa

Gen-ID: 90459

NCBI Accession: NM_153332, NP_699163

UniProt: Q8IV48