ASIC1 Antikörper (N-Term)

Produktnummer ABIN2776229

Der Kaninchen Polyklonal anti-ASIC1 Antikörper (ABIN2776229) detektiert spezifisch ASIC1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Meerschweinchen, Pferd, Kaninchen, Rind (Kuh) und Hund.

Kurzübersicht für ASIC1 Antikörper (N-Term) (ABIN2776229)

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Pferd, Kaninchen, Rind (Kuh), Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASIC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ASIC1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: ACCN2 Antibody - N-terminal region

Sequenz: MELKAEEEEV GGVQPVSIQA FASSSTLHGL AHIFSYERLS LKRALWALCF

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against ACCN2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACCN2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35298-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Andere Bezeichnung: ACCN2

Hintergrund:

Background Information: ACCN2 is the cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ACCN2 is also permeable for Ca2+, Li+ and K+. ACCN2 generates a biphasic current with a fast inactivating and a slow sustained phase. ACCN2 mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. ACCN2 functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. It modulates activity in the circuits underlying innate fear.This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products.

Gene Name: Acid-sensing (proton-gated) ion channel 1

Alternative Symbols: ASIC, ACCN2, BNaC2

Protein Name: Acid-sensing ion channel 1

Molekulargewicht: 65kDa

Gen-ID: 41

NCBI Accession: NP_064423

UniProt: P78348