IDH3A Antikörper (N-Term)

Produktnummer ABIN2775312

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-IDH3A Antikörper wird verwendet zum Nachweis von IDH3A in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Meerschweinchen und Zebrafisch (Danio rerio). Er wurde validiert für WB, IHC und IP.

Kurzübersicht für IDH3A Antikörper (N-Term) (ABIN2775312)

Target: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Meerschweinchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IDH3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-IDH3A Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: IDH3A Antibody - N-terminal region

Sequenz: MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PSEAKESMDK NKMGLKGPLK

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against IDH3A. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IDH3A

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42237-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-IDH3A Antikörper (ABIN2775312)

Lin, Cheng, Tsai, Tsai, Hu, Chang, Yeh, Chen, Liao, Chang: "Loss of the respiratory enzyme citrate synthase directly links the Warburg effect to tumor malignancy." in: Scientific reports, Vol. 2, pp. 785, (2012) (PubMed).

Details zu IDH3A

Target: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Andere Bezeichnung: IDH3A

Hintergrund:

Targeted Subunit: alpha, mitochondrial

Background Information: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

Gene Name: Isocitrate dehydrogenase 3 (NAD+) alpha

Alternative Symbols: RP90

Protein Name: Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Molekulargewicht: 40kDa

Gen-ID: 3419

NCBI Accession: NP_005521

UniProt: P50213