DDX50 Antikörper (N-Term)

Produktnummer ABIN2775257

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DDX50 in WB. Er zeigt eine Reaktivität gegenüber Human, Hund, Rind (Kuh), Meerschweinchen, Pferd, Schwein und Kaninchen und wurde in 1 Publikation erwähnt.

Kurzübersicht für DDX50 Antikörper (N-Term) (ABIN2775257)

Target: DDX50 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 50 (DDX50))

Reaktivität: Human, Hund, Rind (Kuh), Meerschweinchen, Pferd, Schwein, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDX50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DDX50 Antikörper

Bindungsspezifität: N-Term

Sequenz: EESESQKKER QKSDRRKSRH HYDSDEKSET RENGVTDDLD APKAKKSKMK

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Pig: 93%, Rabbit: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against DDX50. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX50

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 737 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-DDX50 Antikörper (ABIN2775257)

Valdez, Yang, Hong, Sequitin: "Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs." in: Gene, Vol. 284, Issue 1-2, pp. 53-61, (2002) (PubMed).

Details zu DDX50

Target: DDX50 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 50 (DDX50))

Andere Bezeichnung: DDX50

Hintergrund: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX50 is a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. DDX50 and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. DDX50 gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.
Alias Symbols: GU2, GUB, RH-II/GuB
Protein Interaction Partner: SUMO3, UBC, LIN28A, PRKRA, TARBP2, EED, RNF2, TARDBP, SRPK2, WHSC1, KRAS, ESR1, BARD1, CAND1, CUL3, HDGF, STAU1, SRRM2,
Protein Size: 737

Molekulargewicht: 81 kDa

Gen-ID: 79009

NCBI Accession: NM_024045, NP_076950

UniProt: Q9BQ39