MCM10 Antikörper (Middle Region)

Produktnummer ABIN2774890

Der Kaninchen Polyklonal Anti-MCM10-Antikörper wurde für WB validiert. Er ist geeignet, MCM10 in Proben von Human, Hund, Rind (Kuh), Kaninchen, Pferd und Schwein zu detektieren.

Kurzübersicht für MCM10 Antikörper (Middle Region) (ABIN2774890)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Reaktivität: Human, Hund, Rind (Kuh), Kaninchen, Pferd, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MCM10 Antikörper

Bindungsspezifität: Middle Region

Sequenz: PKLSALAEAK KLAAITKLRA KGQVLTKTNP NSIKKKQKDP QDILEVKERV

Homologie: Cow: 77%, Dog: 86%, Horse: 93%, Human: 100%, Pig: 77%, Rabbit: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against MCM10. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MCM10

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 874 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu MCM10

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Andere Bezeichnung: MCM10

Hintergrund: This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Alias Symbols: CNA43, MGC126776, PRO2249, DNA43
Protein Interaction Partner: GOLGA2, MCM6, TRIM37, CCND3, ORC3, CDC45, CDC7, UBC, ORC4, ORC2, MCM7, MCM3, MCM2, CDKN2A, CDKN1A, CDK6, CDC6, CDC5L, CCND1, APP, RBX1, VPRBP, CUL4A, DDB1, IKBKG, RPAP1, CEP70, HAUS1, CCDC85B, DPPA2, NINL, ZBTB8B, HOOK2, MCM4, ZBTB43, CEP72, CALCOCO2, KRT
Protein Size: 874

Molekulargewicht: 98 kDa

Gen-ID: 55388

NCBI Accession: NM_018518, NP_060988

UniProt: B7ZKZ8

Pathways: Mitotic G1-G1/S Phases, DNA Replication