RAD18 Antikörper (Middle Region)

Produktnummer ABIN2774765

Dieses Anti-RAD18-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RAD18 in WB. Geeignet für Human, Ratte, Maus, Schwein, Kaninchen, Rind (Kuh), Hund, Pferd und Meerschweinchen. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für RAD18 Antikörper (Middle Region) (ABIN2774765)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reaktivität: Human, Ratte, Maus, Schwein, Kaninchen, Rind (Kuh), Hund, Pferd, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAD18 Antikörper

Bindungsspezifität: Middle Region

Sequenz: KQVTKVDCPV CGVNIPESHI NKHLDSCLSR EEKKESLRSS VHKRKPLPKT

Homologie: Cow: 100%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against RAD18. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAD18

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 495 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-RAD18 Antikörper (ABIN2774765)

Tsuji, Watanabe, Araki, Shinohara, Yamagata, Tsurimoto, Hanaoka, Yamamura, Yamaizumi, Tateishi: "Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, (2008) (PubMed).

Details zu RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Andere Bezeichnung: RAD18

Hintergrund: RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RNF73
Protein Interaction Partner: TAX1BP1, UBC, SHPRH, RAD18, SIVA1, USP2, UBE2B, UBE2A, HLTF, PCNA, BPLF1, USP5, DBF4, BRCA1, DZIP3, POLH, DTL, Ankrd32, MLKL, ECT2, RPL10A, TRIM23, PCGF6, TRIM8, RFC2, CPD, SPRTN, ZMYM2, MAPK8, POLN, WRNIP1, gag-pol, ORC2, SUMO2, POLK, NBN, Smc6, HIST1H2A
Protein Size: 495

Molekulargewicht: 56 kDa

Gen-ID: 56852

NCBI Accession: NM_020165, NP_064550

UniProt: Q9NS91