NEDD4-2 Antikörper (Middle Region)

Produktnummer ABIN2774731

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NEDD4-2 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen und Zebrafisch (Danio rerio).Es wurde in 1 Publikation zitiert.

Kurzübersicht für NEDD4-2 Antikörper (Middle Region) (ABIN2774731)

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD4-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NEDD4-2 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: NEDD4L Antibody - middle region

Sequenz: TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43192-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-NEDD4-2 Antikörper (ABIN2774731)

Raikwar, Thomas: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, (2008) (PubMed).

Details zu NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Andere Bezeichnung: NEDD4L

Hintergrund:

Background Information: NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.

Gene Name: Neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Alternative Symbols: RSP5, PVNH7, NEDD4-2, NEDD4.2, hNEDD4-2

Protein Name: E3 ubiquitin-protein ligase NEDD4-like

Molekulargewicht: 110 kDa

Gen-ID: 23327

NCBI Accession: NP_056092

UniProt: Q96PU5

Pathways: Negative Regulation of Transporter Activity